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KMID : 0900919960200040413
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Korean journal of Animal Reproduction
1996 Volume.20 No. 4 p.413 ~ p.421
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Tendency and Problems in Porcine in-vitro Fertilization
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Park Choon-Keun
Kim Choung-Ik
Cheong Hee-Tae
Yang Boo-Keun
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Abstract
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In vitro culture has provided new information on the mechanisms involved in fertilization how sperm and oocyte fuse together. At the same time, results obtained in vitro have led to new questions. Techniques for In vitro maturation of porcine oocytes have progressed such that the problem of the low rate of pronucleus formation with in vitro matured oocytes after in vitro fertilization has been nearly improved. On the other hand, porcine spermatozoa have been shown to be capacitated if the fertilization medium contains caffeine and Ca^2+, but the incidence of polyspermy in IVM-IVF oocytes is still high. To prevent polyspermy, co-culture with oviductal cells, sperm preincubation with porcine follicular fluid or control of sperm concentration, have been examined with significant effects but still remarkably high rates of polyspermy. The under standing of these influences is a prerequisite to enhancing in vitro production of porcine em bryos.
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